Journal: BMC neuroscience
Article Title: Pro-cognitive efficacy of Prospekta in a rat model of age-associated cognitive impairment.
doi: 10.1186/s12868-025-00958-4
Figure Lengend Snippet: Fig. 9 Expression of selected biomarkers of aging in the brains of aged rats. Data are presented as m ± SE. Results of densitometric analysis of bands on grayscale images expressed as the ratio of absorbance of NR1-, NR2B-, Iba1-, GABAA-, DRD1-, and TrkA-positive bands over bands stained with antibodies to GAPDH. Intergroup differences were assessed with the Student’s t-test (for TrkA expression, the Welch’s t-test was used). Iba-1 lev els were analyzed by the Mann-Whitney U test due to a non-normal data distribution
Article Snippet: Antibodies to detect the proteins of interest are listed below: NMDA receptor NR1 subunit (mouse monoclonal, ref. ASM10244 Absepta), NMDA receptor NR2B subunit (mouse monoclonal, ref. 05-920 MerckMillipore), dopamine receptor (DRD1) (rabbit polyclonal, ref. 720276 Thermo Fisher Scientific), tyrosine kinase-A (TrkA) receptor (rabbit polyclonal, ref. 06-574 Merck-Millipore), gamma-aminobutyric acid (GABA-A) receptor α1 subunit (mouse monoclonal, ref. ASM10222 Absepta), actin-binding protein (Iba1) (goat polyclonal, ref. AHP2024 Bio-Rad).
Techniques: Expressing, Staining, MANN-WHITNEY